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Then, the circular region is filled by the morphological operation, which is shown in Fig. 2(d).
To do this, a whole slide image was used to produce a supported image by the morphological operation of opening and brightness equalization.
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As shown in the grayscale image of two adjacent cells (Figure 6a), the two cells were considered as one blob after the morphological operation (dilation followed by erosion (Figure 6b,c), and therefore, the center of the cells was found at the wrong position as shown in Figure 6d.
Then, we reduce the noise with the spatial filter and the morphological operation.
Figure 3 shows the flowchart algorithm of skull stripping by using the morphological operations.
Second, they extract the defect by applying the morphological operations which eliminate small holes, spots, and connect the closely regions.
The morphological operations also can be implemented as one of the ways to detect the macula.
Binary and gray scale images have been considered in the morphological operations of skull stripping.
After applying the morphological operations, we remove pits with an area equal to or above A max = 100 pixels as these are in most cases caused by ridges.
Since in some cases I B contains small spurs or isolated pixels (a white pixel surrounded by black pixels in the respective 3 × 3 pixel neighborhood) which do not belong to pits, we post-process the binary image by applying the following morphological operations (the actual implementations of these operations are provided by the MATLAB function bwmorph): Spur removal This step removes spur pixels.
Although these discrete parts can get connected by conducting morphological operation in Figure 9f, the shape distortion of the target is rather serious.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com