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The sample to be separated is injected at the beginning of the column and is transported through the system by the mobile phase.
At first, only adsorptive solids were used as the stationary phase, because liquid coatings were swept away by the mobile phase.
The literature describes several special descriptors used for chromatographic retention modeling, all of these do not describe the screening of electrostatic potential by the mobile phase in use.
During the CCC separation, the extraction solution was continuously fed in the sample loop by turning the ten-port valve; the extraction solution was then pumped into the CPC column and eluted by the mobile phase of the two-phase solvent system mentioned above.
The drugs were extracted by the mobile phase with shaking and filtration.
Due to the viscous nature of ethylene glycol, time taken by the mobile phase M7 was too much (4 h).
Similar(48)
The optimized conditions for the separation of the eluents were achieved by using the mobile phase of methanol/water 90:10 monitored by UV detection 240 nm at a flow rate of 1 ml min−1 with pH 3.5 adjusted by orthophosphoric acid.
Quantification of solanesol was achieved by optimizing the mobile phase at 60/40 acetonitrile isopropanol and lowering the oven temperature to 22 °C using a standard reverse-phase high performance liquid chromatography (RP-HPLC).
Selectivity of the method was evaluated by injecting the mobile phase, standard drug solution, and tablet extract.
Other trials were made by changing the mobile phase pH (3 9) using phosphoric acid, glacial acetic acid or triethyl amine, however, in vain.
The HPLC retention time of the sphingoid base 1-phosphates could be shifted by adjusting the mobile phase to pH 5.5, which is useful in separating overlapping compounds (such as sphingosine 1-phosphate and 4-D-hydroxysphinganine) and in confirming the identity of sphingoid base 1-phosphates in biological samples.
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