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In the first case, the complexes were strongly affected by the missing wedge.
However, we are aware that these tomograms are still affected by the missing wedge along the z direction, as pointed out by the reviewers.
Once a tomogram has been calculated, interpretation of the 3D volume can be difficult due to the high noise level and limited resolution of tomograms and the distortions introduced by the missing wedge.
The potential artifacts that are caused by the missing wedge in the EM data could have affected this particular conclusion, especially the connections between the filaments, as the authors also showed in their simulations.
The lack of detail in the membrane region is unlikely to be caused by the missing wedge as we have observed a similar phenomenon in sub-tomogram averages of other membrane proteins, which have no missing wedge of information, and in single-particle maps when the resolution does not extend beyond 15 Å.
A classification technique for subtomograms based on 2D reprojections has been proposed to overcome the uncertainties in classification introduced by the missing wedge (Yu et al. 2010, 2013).
Similar(50)
We show that the missing wedge causes a bias in classification, and that this bias can be minimized by supplementation with data from the Fourier transform of the averaged structure.
Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology.
Due to the missing wedge artifact, thylakoid grana that may have been present at the top and bottom of the chloroplast could not be resolved.
The missing wedge effect leads to distortions of the density maps in real spaces.
The missing wedge effect leads to distortions of the density maps along the tilt-axis.
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