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These analyses confirmed the monotonic expression changes with mosquito age as predicted by the microarray results.
The two cell populations are also known to release different neurotransmitters, which is confirmed by the microarray results.
This view is reinforced by the microarray results, that uncover considerable similarities in gene expression profiles of IRP2Δ73 and control tumors.
Both THP-1 cells and primary human monocytes were used to directly test the association of CO suppression with NF-κB signaling as suggested by the microarray results.
This idea is supported by the microarray results as several other genes involved in electron transport were also differentially expressed.
Selected differentially expressed genes identified by the microarray results were then confirmed using quantitative reverse transcription PCR (qRT-PCR).
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Interestingly, by comparing the microarray results obtained from GN-11 neurons and MEFs analyses we observed opposite modulations for genes involved in proliferation (i.e. Cdc2a, Skp2).
Of particular interest, elevated CCL21 protein levels were detected by IHC in Chrnb2−/−mutant retinas at P4, as predicted by the microarray result, but not in adult mutant or WT retina, also consistent with the microarray result (Figure 5D).
As shown in Figure 2A, the LDL-R, the HMG-CoA reductase, the FPPS, and the fatty acyl synthase (FAS) genes were all induced by lovastatin, but not by docetaxel, confirming the microarray results.
Therefore, the analysis of selected miRNAs by qPCR confirmed the microarray results, indicating the quality of the miRNA microarray.
The expression of the 3 genes in the muscle and 3 genes in the liver measured by qPCR confirmed the microarray results.
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