Sentence examples for by the microarray probes from inspiring English sources

In both root and shoot samples, about 95% of transcripts were supported by the microarray probes.

Instead they indicate hybridization differences caused by sequence polymorphisms in the mRNA region that is targeted by the microarray probes.

Among detoxifying Ugts, the highly homologous genes of the Ugt1a subfamily could not be distinguished by the microarray probes.

An exception was members of the SSX gene family, which were poorly distinguished by the microarray probes, and of which only SSX1 was confirmed by real-time PCR.

In addition, the genes important for understanding the biological processes involved in metastatic transformation were not selected based solely on the difference in the signal emitted by the microarray probes.

The microarrays were scanned using an Agilent DNA Microarray Scanner G2539A ver. C. 75 percentile normalization was performed for the signals generated by the microarray probes according to the Agilent data analysis protocol.

The hypothesis made was that we would be able to capture the underlying common biological processes related to the intensity of FDG uptake shared by different solid tumors by selecting the microarray probes most strongly correlated with FDG uptake (by means of univariate linear regression) in order to build a predictive signature (see Additional file 1 for full details).

The genomic coverage of ChIP-chip is limited by the microarray probe design, and the coverage of ChIP-seq is dependent on sequencing depth.

The Q-RT-PCR assays were designed to specifically amplify a region of the transcript different from that covered by the microarray probe in order to obtain an independent measurement.

Further, as most C. briggsae mRNAs are not bound by GLD-1, the microarray probes for most gene products will have near-zero fluorescent measurements, which violates the assumption that most genes between treatments and controls are not differentially expressed and are also normally distributed (supplementary fig. S3, Supplementary Material online).

In order to exclude the possibility that the observed differences in transcript levels are caused by suboptimal hybridisation of the microarray probes or the PCR primers due to polymorphisms in different parasite genotypes, we sequenced selected gene fragments from different cell lines.