Sentence examples for by the microarray platforms from inspiring English sources
Solexa/Illumina deep sequencing has the highest overall power followed by the microarray platforms Agilent and Affymetrix.
So the largest effects found in the low-intensity range in this study are duly detected by Solexa/Illumina, but missed by the microarray platforms.
For this purpose, sequences of unigenes that were represented by the microarray platforms were used to blast against sequences of a potato unigene database (i.e. TIGR consensus build of potato, version 10.0).
Overall over 90 percent of the miRNA microarray results tested were validated by qPCR, confirming the integrity of the data generated by the microarray platform.
Probes and hybridizations were performed by the Microarray platform of the IGBMC and Génopole Alsace-Lorraine (http://www-microarrays.u-strasbg.fr).fr
In addition, real-time kinetic PCR analysis of selected genes also verified the differential regulation as identified by the microarray platform.
The difference is probably also accentuated by the competitive pair-wise comparison of samples used by the microarray platform in this study compared with the individual gene/ GAPDH CT expression ratio values generated using real-time PCR.
The well-known hypoxia responsive miR-210-3p miR-210-3p miR-210-3p detecouldby the microarray platform most likely for technotal reasons, however, its upregulation upon hypoxic stress was apparent be qPCreliably
Overall, these findings imply the global transcriptional readout in the transfected C2C12 cells described by the microarray platform can be attributed to the impact of the transfection of the Rnf14 variants.
121 miRNAs present in both microarray platforms had no detectable measure by small RNA-seq in any of the three biological replicates, whereas 147 detected by small RNA-seq had not been addressed by any of the microarray platforms.
