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Hybridization and scanning was done by the microarray facility in the University of Pennsylvania.
The protocol was performed by the Microarray Facility at the Institute of Molecular and Cell Biology in Singapore.
In short, Operon's long-oligonucleotide spotted arrays covering the whole known genome represented by 36,000 genes and gene transcripts were used (printed by the microarray facility at KTH, Royal Institute of Technology, Stockholm, Sweden).
Gene microarrays of 34 580 (70-mer) human oligos representing 24 650 genes and 37 123 gene transcripts printed on aminosilane-coated microarray slides were supplied by the microarray facility of the Prostate Centre at Vancouver General Hospital (Vancouver, Canada), and microarray analysis was performed as described earlier (Snoek et al, 2009).
PCR amplified cDNA elements derived from pineapple fruit, root tip, root and nematode infected roots and gall cDNA libraries were printed in duplicate onto Corning UltraGAPS slides by the Microarray Facility at the ARC Special Research Centre for Functional and Applied Genomics, University of Queensland [ 49].
Gains and losses of the genes identified in the cDNA microarray analysis were assessed using genomic array slides produced by the Microarray Facility at Health Enterprise Rikshospitalet-Radiumhospitalet, containing 5000 unique BAC clones (RPCI-11 library, Wellcome Trust Sanger Institute, Cambridge, UK) that covered the whole genome with a resolution of approximately 1 Mbp.
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Maintenance of the microarray facility was supported by the High-Tech Research Center at Kanazawa Medical University.
RNA integrity was checked by agarose electrophoresis at the source laboratory and again at the microarray facility.
We thank the microarray facility at Roswell Park Cancer institute for their work.
UC provided the microarray facility.
BB analyzed microarray data by IPA HS, and ODB generated the microarray and the qRT-PCR data, MK provided the microarray facility, TT participated in the design of the study, contributed to the data analysis, and wrote and finalized manuscript.
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