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The QPCR results were consistent with the results obtained by the microarray experiments (Fig. 3).
Of the 29 putative Pat substrates identified by the microarray experiments, 22 were isolated using the ASKA collection [23].
Poor expressional correlation among the rest of the ERG orthologs also suggests that the corresponding proteins either have lost their ability to physically interact in order to form stable complexes, or they do so in conditions/moments not covered by the microarray experiments explored here.
The resulting time course profiles are highly correlated to those obtained by the microarray experiments (Pearson correlation between 0.74 and 0.93; see Additional File 1).
In order to validate a subset of probes identified by the microarray experiments to harbor SNPs, we performed reverse transcription-PCR.
Two other genes shown by the microarray experiments to be up-regulated within the rhizome of ginger or turmeric, respectively, were the MADS box genes GT_01880 and GT_15843.
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The missing values in the microarray dataset are traditionally estimated by repeating the microarray experiments or simply replacing the missing values with zero or the row average (the average expression over the experimental conditions).
One cycle difference in the RT-PCR experiments corresponds to the two-fold difference in gene transcripts between the NILs examined by the microarray experiment.
Cluster analysis, as first reported by Grant et al. (2000), was performed to determine if candidate genes identified by the microarray experiment were randomly distributed across the genome.
For 15 of the selected genes the log2 FC values for tebuconazole treatment obtained by the microarray experiment were coherent with those of qRT-PCRs.
The approach described herein does not assign patients' transcriptomes (interrogated by the microarray experiment) to a diagnostic category (DM2 vs. IGT vs. NGT or normal).
More suggestions(17)
by the laboratory experiments
by the microarray analyses
by the confirmation experiments
by the microarray results
by the docking experiments
by the diffusion experiments
by the breakthrough experiments
by the microarray data
by the microarray studies
by the transmigration experiments
by the field experiments
by the nanoindentation experiments
by the evaluation experiments
by the column experiments
by the reusability experiments
by the interference experiments
by the xenograft experiments
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