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The expression of 5HT3A gene was somewhat under-estimated by the microarray data.
This gene was also up-regulated in the African-American tumors of this study, as indicated by the microarray data.
As predicted by the microarray data, in situ hybridization signal for Gpr126 was no longer detectable in the adult cerebellum (Fig. 4, right panels).
The gene list that was generated by the microarray data was imported into Pathway Studio to identify and group genes into specific biopathways.
Other data supporting the idea of p50 and Bcl-3 working together to effect gene expression changes with unloading is that all of the p50 targets revealed by the microarray data were also Bcl-3 target genes.
More importantly, as a validation of the two-step normalization, the magnitude of the changes as assessed by quantitative RT-PCR agreed very closely with the magnitude of changes suggested by the microarray data.
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The three remaining CC199 isolates tested in the chinchilla model of pneumococcal disease were screened for the presence or absence of these genetic regions, both by PCR and by examining the microarray data.
By analyzing the microarray data, we found that the expression of EPB41L4A-AS2 wasignificantlylowerwer (Student's t-test, P = 0.042, Fig. 5D) in the resistant group than the non-resistant group.
Therefore, we determined the differences between clusters A and B by comparing the microarray data of the two subclusters.
Auxin-responsive genes from A. thaliana and O. sativa were identified by analysing the microarray data from auxin treatment experiments.
We assessed the frequency of false-positive DE genes in our Affymetrix dataset by validating the microarray data using two independent methodologies.
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