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Microarray analysis was performed by the Microarray Core at the Mount Sinai School of Medicine.
Steps outlined in this section were performed by the microarray core facility at this institution.
Verification of RNA integrity and microarray analysis were performed by the Microarray Core Facility at the Mount Sinai School of Medicine.
Total mRNA was isolated using the RNAeasy Mini Kit (Qiagen) and hybridisation to DNA microarray was performed by the Microarray Core.
Affymetrix labeling, hybridization, staining, washing, scanning and statistical analysis were done by the Microarray Core at Baylor College of Medicine (http://www.bcm.edu/mcfweb/).edu/mcfweb/
Expression screening experiments using Affymetrix GeneChip HG-U133 plus 2.0 (Santa Clara, CA, USA) were carried out by the Microarray Core at the Louisiana State University Health Sciences Center, New Orleans.
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We also acknowledge the outstanding microarray hybridization and scanning services carried out by Gigi Ostrow, scientific coordinator of the microarray core facility at the ICBR Interdisciplinary Center for Biotechnology Researchh) of the University of Florida.
All arrays were labeled, hybridized, and processed in the Microarray Core Facility at Shanghai Children's Medical Center using conditions recommended by the manufacturer.
The Microarray Core Facility of University of Texas Southwestern Medical Center performed the microarray analysis.
Both target preparation and hybridization were performed in the Microarray Core Facility, University of Kentucky.
Affymetrix microarrays were processed in the Microarray Core Facility of the Institute of Research of Biotherapy, CHRU-INSERM-UM1 Montpellier http://irb.chu-montpellier.fr/ by Veronique Pantesco.
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