(a) Analysis of mRNA expression in human PrCa samples24 of a selection of putative HSF2 target genes identified by the microarray analysis (Figure 5).
We previously reported that OsAP77 was PBZ inducible by the microarray analysis (Shimono et al. [2003]).
Some of the DEGs detected by the microarray analysis were also analyzed by RT-PCR, and the gene responses detected by the microarray analysis were confirmed by the RT-PCR results (Additional file 2: Figure S1). Figure 1 Similarity of gene responses across different fog treatments.
On the other hand, gene expression data obtained by the microarray analysis are usually moderately correlated with the expression levels of corresponding protein products due to the post-transcriptional regulation.
The results showed that relative expression levels obtained from the qRT-PCR analysis correlated well with those produced by the microarray analysis (Supplementary Figure S1).
In the case of Avp, Oxt and Trh, the aFD value measured by qPCR was considerably larger than that generated by the microarray analysis.
To challenge this hypothesis we started by confirming the effects of RA on the Erk pathway as suggested by the microarray analysis.
We have validated the pregnancy-specificity of 7 transcripts with differences in expression between pregnant and non-pregnant whole blood as determined by the microarray analysis.
Immunoblotting was used to examine the effect of PDGF-BB treatment at the protein-level for several of the genes identified by the microarray analysis.
Biological networks captured by the different microarray platforms were generated using IPA and scored based on the relationship between the total number of genes in the specific network and the total number of genes identified by the microarray analysis.
We selected a number of these genes for further analysis based, in part, upon their high levels of expression in discrete prominences as determined by the microarray analysis (Figure S9).
