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We thus used quantitative PCR to confirm changes in the METH-responsive ER genes identified by the microarray analyses.
We used quantitative PCR to validate the expression of some of the genes identified by the microarray analyses using RNA from individual animals from the four groups.
Eight of them were later shown to be not expressed by the microarray analyses.
Quantitative PCR assays were able to reproduce 60 100% of expression patterns of each gene examined that had been revealed by the microarray analyses (Table 3).
The Msl1-, or Nsl1-KD affected genes determined by the microarray analyses were then confirmed by RT-qPCR under Msl1-, or Nsl1-KD conditions.
We found that another candidate gene identified by the microarray analyses, lola, plays a prominent role in the regulation of GluR abundance.
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17, 18 Collating the genomic analyses can also provide further information; e.g., an additional five genes were added to the integrin-linked kinase (ILK) signaling canonical pathway (identified by RNA-Seq) by overlaying the microarray analyses (Fig. 4).
This was strengthened by findings in the microarray analyses of livers from resveratrol-treated mice, in which downregulation of three cytochrome P450 enzymes was observed (Baur et al 2006).
Further SigH-dependent promoters were searched for within the 5´-UTRs of the genes, which exhibited enhanced transcription in the Δ rshA strain in the microarray analyses, by motif searches using the program Bioprospector [ 35].
Interestingly, we found significant increases in Arc, Egr2, and c-fos mRNA by quantitative PCR but not on the microarray analyses.
The microarray analyses were done by Almac Diagnostics, Durham, NC.
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by the stability analyses
by the trend analyses
by the regression analyses
by the shotgun analyses
by the microarray results
by the macroarray analyses
by the constraint analyses
by the cluster analyses
by the microarray data
by the microarray experiments
by the microarray studies
by the correlation analyses
by the factor analyses
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