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However, the degree of differential expression revealed by the macroarray analyses was typically <2-fold.
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Although neonate selection was discontinued after five days, resistance ratios obtained using both neonates and late instar larvae show that resistance was maintained during the entire larval stage, including the larval instar used in the macroarray analyses.
Additionally, by using macroarray analyses to compare the transcripts present in juicy fruits with those from wooliness, we have previously identified a number of genes that are differentially expressed in these fruits [ 12].
We have previously reported a decreased transcript level of pectate lyase when woolly fruits were compared to juicy fruits by macroarray analyses [ 12].
A similar conclusion can be reached from the micro- and macroarray analyses of transcripts detected in adult honey bee workers performing different tasks during their adult life cycle [ 6, 7].
However, the high correlation of the expression ratio (R = 0.90) between real-time RT PCR and macroarray analyses showed the reliability of macroarray analyses with the validity of selection method for putatively induced genes.
Further experiments for validation were conducted on a subset of candidate genes selected from both the subtraction library and macroarray analyses.
But, a gene encoding rhtB family transporter (bll7341 in LOS 22) was unsubstantially repressed by using real-time RT PCR, although the clone covering this gene was significantly down-regulated in macroarray analyses.
Relative expression levels seem to differ between real-time RT PCR and macroarray analyses to some extent.
Thirty-four grapevine samples from various origins were tested by the macroarray, RT-PCR and ELISA.
Thirty were positive for virus infection using RT-PCR, 28 by ELISA and 25 by the macroarray.
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