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A cloning-expression system was developed to assemble and test 54 unnatural trimodular PKSs flanked by the loading module and the thioesterase from the erythromycin synthase.
The glutarimide starter unit is assembled by the loading module of LtmCD and PKS module-2 and -3 of LtmE.
This led to the conclusion that the TEII is involved in the control of the starter unit by the loading module (Fig. 1c).
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Utilizing the E. coli platform, Pfeifer et al. (2001) were able to generate an analogue of 6-dEB by replacing the loading module with that from rif PKS.
This is further highlighted by an intra-species comparison of the domain structure of the load modules of MlsA and MlsB.
This is realised by the load-prediction module [4].
Initially, the representation of the resource load conditions is computed based on the measured raw data of the network response time--this is realised by the load-tracker module.
The aerosol loading module was comprehensively updated by CH14.
The choice of starter unit is governed by the substrate specificity of a distinct loading module.
The core macrocyclic lactone of mycolactone E is synthesised by MlsA1 and MlsA2, comprising a loading module and nine extension modules, terminating in a putative integral C-terminal thioesterase.
Our aerosol loading module is referred to as the Aerosol Loading Interface for Cloud microphysics In Simulation (ALICIS).
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