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Our current observations argue that the differences in fresh samples might originate from differential susceptibilities to death under various pathological conditions, which are accentuated by the isolation procedure.
Slices were maintained in vitro for a minimum of 3 days prior to use, a period at least over which tissues recover from experimental trauma caused by the isolation procedure [48].
In the absence of activating signals and TGF-beta1, spontaneous activation of p38 MAP kinase as well as Smad 3 was observed (Figure S 1), which may have been caused by the isolation procedure and/or cytokines carried over into the culture.
All RNA was pure and not degraded by the isolation procedure (data not shown).
This may be due to the fact that, in the hepatic glycogen proteome, endoplasmic proteins were also identified by the isolation procedure.
Cells were left over night at 37°C in an atmosphere with 5% CO2 to differentiate into macrophages and to minimize possible gene expression changes associated with cell stress caused by the isolation procedure.
Similar(50)
This observation may indicate that the HF phenotype develops relatively early in response to cardiac stress, that it is rather non-specific and/or, at least in part, an artefact introduced by the isolation procedures from fibrotic myectomy samples.
STRO-3-positive, human bone marrow-derived MPCs (Batch# M111sP4) were prepared by Lonza (Walkersville, MD, USA) for Mesoblast Ltd (Melbourne, Australia), according to the isolation procedure originally described by Gronthos and colleagues [ 9, 13].
The sensitivity of antibiotic resistance surveys can be significantly enhanced by including a selective step in the isolation procedure.
In two independent reports, Soldati et al. characterized phagosomes from the amoeba Dictyostelium discoideum using a modified version of the isolation procedure described by Desjardins et al. (1994b).
We attempted to reduce differences originating from the isolation procedure per se by exposure of the mixed cultures to the same processing technique without performing the separation.
More suggestions(15)
by the averaging procedure
by the pinealectomy procedure
by the isolation habitat
by the sampling procedure
by the bootstrapping procedure
by the iterative procedure
by the randomization procedure
by the isolation property
by the transplantation procedure
by the l1l2 procedure
by the staircase procedure
by the preparation procedure
by the clustering procedure
by the matching procedure
by the learning procedure
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