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All four loci of lost genes were flanked by the insertion sequence element IS481.
M. microti strains yield broadly similar, high copy number fingerprints by the insertion sequence 6110 based restriction fragment length polymorphism method (IS 6110 RFLP) (7 ).
The 2 sequenced serogroup C and serogroup Y isolates harbored functional cssE/cssF genes with an IS1301 transposase adjacent to cssF in serogroup Y. Serogroup W cssF was interrupted by the insertion sequence IS1301.
Further sequencing of the IS 1999 located upstream of blaOXA-48 showed that it was consistently truncated by the insertion sequence IS 1R, as initially described in Turkey and more recently in Lebanon and Egypt (2, 8 ).
IS6110 insertion sequences are located immediately adjacent to the flanking regions of all the inversion points, suggesting that the genome rearrangements observed in these 3 strains were driven by the insertion sequence.
Fifteen of the remaining 19 ST-23 isolates harbored frame-shifted alleles (1 mutation IV, 13 mutation V, and 1 mutation VI [ 15]), whereas another was interrupted by the insertion sequence IS1301 (mutation I) (16 ).
Similar(54)
M. avium strains differ from M. intracellulare by containing the insertion sequence (IS) IS 1245 (3 ) and are further discriminated by terms of IS 901 (4 ).
Restriction fragment length polymorphism (RFLP) typing by using the insertion sequence IS 6110 as a probe for strain differentiation is the most widely applied DNA fingerprinting method to study the epidemiology of tuberculosis (1 ).
Previous sequence comparisons using BLAST analyses indicated that one mal operon (the ancestral mal1 operon, mal1An, along with the ancestral malK2An gene in Figure 1) transferred from an ancestor of Tc. litoralis to an ancestor of P. furiosus, likely involving a transposition mediated by the insertion sequences now found in P. furiosus [ 8].
In IS 6110 RFLP patterns, changes can also be accounted for by transposition of the insertion sequence itself.
A sample was sent to the Institute of Tropical Medicine in Antwerp, Belgium for PCR testing for M. ulcerans by detection of the insertion sequence 2404 IS 24044), which was positive.
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