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In the case of clade I with the isolates FnnR-001-04, -004-06, 017-05 anew017-05, the new generations of cod used for restocking after initial outbreak were probably infected by the initial isolate in the population at that site.
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Drug susceptibilities reported were those obtained from the initial isolate available and represent initial resistance.
If more than one isolate were obtained from a patient, only the initial isolate was submitted.
When >1 isolate was recovered from the same person, only the initial isolate was included in the study.
Therefore, after the initial isolate, successive isolates for the same patient during the next 90 days were excluded.
The duplicate isolate was examined if a viable Neisseria sp. was not obtained from the initial isolate.
The initial isolate-(SaB1) was fully vancomycin susceptible (VSSA).
For the initial isolation of putative V. harveyi, isolates were incubated on thiosulfate citrate bile salt sucrose agar plates for 24 h, followed by selection of greenish colonies.
Seventy-four clisolatessobtainedobtained from 52 patients (including the index case), and 10 environmental isolates showed sufficient genomic similarity to the outbreak reference strain (≤8 SNVs different) to be ruled into the outbreak (Table 1).> -wrap-foot> Patients were assigned to the outbreak if an initial isolate was shown by PFGE to belong to pulsotype 27.
Implementation of universal genotyping in New York City consisted, briefly, of 1) requiring submission of the initial positive isolate, reinforced by health code amendment (18, 19 ); 2) advising all relevant laboratories and providers of new requirements; 3) modifying laboratory submission forms; 4) establishing a specimen transport system; and 5) tracking and reviewing all submissions.
Each bap-negative isolate matched the PFGE genotype and antimicrobial susceptibility phenotype of the initial infecting isolate.
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