Sentence examples for by the initial fluorescence from inspiring English sources

Fluorescent intensity at a given time was divided by the initial fluorescence within each region of interest and expressed as F/ F0 SEM.

Fold changes were determined by dividing the final fluorescence by the initial fluorescence.

(D ) Average Annexin-V fluorescence divided by the initial fluorescence ± SEM for the same cells as in C (n = 5).

Maximal amplitude of caffeine-induced peak fluorescence was normalized by the initial fluorescence value (F/F0) and considered as an index of total SR Ca2+ load.

Changes in GCaMP3 fluorescence were normalized by dividing the maximum GCaMP3 fluorescence value by the initial fluorescence basal level monitored before DNP-BSA addition.

pHluorin-LAMP1 fluorescence intensity was quantified in endoysosomes that coexpressed mCherry-OCA2 and pHluorin-LAMP1 by subtracting the initial fluorescence from fluorescence elicited by treatment with BafA1: [(FBafA1 − Fo)/Fo]−1.

Briefly, the relative transcript abundance for each gene at each time point for each replicate was obtained by normalizing the initial fluorescence (IO) values of a gene to 18S rRNA IO values [ 7, 61].

These experiments indicate that TDP-43 is highly mobile within neurites, as indicated by a 55 64% recovery of the initial fluorescence intensity (Fig. 4I).

The melting temperature or Tm was calculated using the StepOne plus software (Life Technologies), based on the initial fluorescence curve by plotting the negative derivative of fluorescence-reporter (−Rn) over temperature versus temperature (−Rn/T).

For the analysis, 20 images were acquired with an interval of 500 ms. The first image captured prior to photobleaching was used to calculate the initial fluorescence intensity followed by 19 consecutive images, in which bleaching and recovery were recorded.

The values were then plotted by normalizing each titration point (F i ) to the initial fluorescence (F0) resulting in a relative fluorescence change (Frel = F i / F0).