The average radial growth rate of the colonies (V r ) was calculated using the following formula: {V}_r=frac{R_1-{R}_0}{n}, mathrm{mm}/mathrm{day}, where R 1 is colony radius in the late phase of linear growth (limited by the size of the Petri dish), in mm; R 0 is the initial colony radius at the early stage of linear growth, in mm; and n is the duration of the linear growth, in days.
The proposed algorithms are also found to be relatively insensitive to the initial colony and size of the colony used compared to the original algorithm.
In addition, an estimated 7 to 9 population doublings took place during the initial colony formation.
By 24 months, the survival of αMUPA was almost 95%, while only about 55% of WT mice from the initial colony survived.
end{aligned} (8 First, the initial colonies are divided based on the power of the empire.
The initial colonies would be built at the Earth-Moon and Lagrange points.
Much of the variation we observed in colony growth was likely driven by initial colony size.
For each interaction tested, measurements were done in quadruplicate by picking four initial colonies into separate cultures and performing the assay for each culture.
Of the 90 initial colonies transplanted, 59 were recovered for sclerite analysis.
CCL2-KO mice (initial colony kindly provided by Dr Barrett Rollins, Department of Medical Oncology, Harvard University, Boston, MA, U.S.A ., backcrossed with WT (wild-type) Balb/C mice for greater than ten generations, have been described previously [ 16].
