The Baz1b results were reproduced in two independent sets of pooled samples, and by testing the individual samples separately.
We recognize that the regions identified could still be limited by the individual sample size of the histotypes.
The amounts of CBR1 protein and GSTA protein were detected by immunoblotting both in the individual samples and in the pooled samples.
Dimensional reduction of the salivary profile data by principal component analysis (PCA) explained 45% of the total variance among the individual samples by the first three components.
According to the idea of learning with label proportions [9 11], we can learn a model to predict labels of the individual samples by grouping the training samples and providing proportions of the labels in each group.
It is noteworthy that only 50 of the 62 MAP-positive pools (80.6%) were confirmed by isolation of MAP from the individual samples.
The two dominant phyla for UL, ML and DL samples were the same but with different relative abundance, which were Proteobacteria with 40.4 56.1%, followed by Nitrospirae with 11.6 23.9% in the individual samples (Additional file 1: Figure S3A).
For each sample, gene expression ratio values were calculated by comparing the individual sample (tumor or normal) with the respective common baseline.
In the individual sampling-model by Kielhorn et al. [61] patients start after inadequate response to two biological DMARDs and begin with states related to a certain treatment sequence.
