Moreover, by optimizing the imaging analysis, we reduced the scanning time of a 384-well plate to ∼30 minutes.
The algorithm applied for segmentation of the nucleus, cytoplasm, and primary cilium in SEMG was confirmed by the manual imaging analysis in both serum positive and negative conditions.
The total size of GA was measured in the processed FAF images by automated imaging analysis software that uses region-growing techniques to segment the areas of GA. [42] This procedure significantly reduced the estimated within-group error variance from 0.761 mm2/year, 95% CI [0.645; 0.898] previously [32] to 0.212 mm2/year, 95% CI [0.182; 0.246].
Photographs obtained under microscopy were used to calculate the collagen volume fraction of the myocardial interstitium by computer imaging analysis system (MPIAS 500, Japan).
We wish to acknowledge the help provided by staffs for Confocal imaging analysis, TC5B Bio-imaging tools, Technology Commons, College of Life Science, NTU.
Therefore, we investigated whether the Cry1Aa/CryR ablation system was applicable to non-proliferating tissues, such as the fat body, by live imaging analysis (Fig. 4b, Additional file 5).
The effect of lamin A/C down-regulation on nuclear size (diameter of the long axis) was quantified by computer-assisted imaging analysis, and the results indicated that lamin A/C down-regulation caused a general increase in nuclear size.
Funding for the software used for CT imaging analysis was provided by the Canadian Foundation for Innovation (CFI).
The endothelial nature of this staining pattern was confirmed by double immunohistochemistry for the IR and CD31, aided by spectral imaging analysis.
We extracted data only from the corresponding brain areas by the imaging-based analysis.
Descriptive characteristics, focusing on the ratio of X-Ai to lung volume by CT imaging analysis, were provided in Table 3 and graphed in Figure 2.
