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GOTM reports only those enrichments that are statistically significant as determined by the hypergeometric test.
The enrichment score of one protein's neighbors on a STRING network was defined as the −log10 of the p-value generated by the hypergeometric test.
We note that, while the value n1 is the test statistic considered by the hypergeometric test, the value of n1 is directly proportional to n1/n2, and thus we view this approach as a test of the n1/n2 ratio.
For all the over-represented or under-represented functional features detected by the Hypergeometric test, if the candidate has the feature it was represented by 1, otherwise it was assigned 0. SVMs were used to evaluate the effects of different kinds of features.
Now we determine (by the hypergeometric test [48], [63] p(M,G,W), the p-value for over-representation, i.e. the probability of finding more than N M,G,W) hits for motif M within window W for |G| genes selected at random from the 8,110.
Each P value is computed by the hypergeometric test.
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Statistical significance of the enrichment data was estimated by means of confidence p-values calculated by applying the hypergeometric test and Bonferroni correction.
Enrichment for differential methylation was determined by applying the hypergeometric test to the overlap between known gene sets and those found in our study to be differentially methylated.
An assessment of significantly enriched processes networks for differentially expressed proteins was performed by evaluating the probability of a random intersection between the differentially expressed proteins with functional processes by applying the hypergeometric test.
Of those 37 genes that we were able to annotate, no significant enrichment was noted by using the hypergeometric test (P < 0.05), relative to the background set of all Enallagma GO annotations.
Then by using the hypergeometric test to calculate the association scores of all gene pairs in yeast, we constructed seven biological associations including TFB association, TFR association, MP association, FA association, PI association, GI association, and LE association.
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