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The predicted protein, Sk-ARP1 (for S. kunkelii adhesion related protein 1), encoded by the first sequence, Sk-arp1, contains seven rather than six sarpin repeats and has C-terminal domains resembling those of SARP1 [ 15].
We also defined the beginning of the academic year by the first sequence of procedures carried out by trainees after their initial contact with the hospital rather than by a specific calendar month.
Two cases that were wildtype using Sanger sequencing and NGS and showed borderline results in HRM exhibited a p.G596 mutation using pyrosequencing with a mutation frequency of 8 and 14% (case 29 and 39, Additional file 1) analyzed by the first sequence to analyze.
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Sequences were aligned to an in silico bisulfite-converted human genome using the BS Seeker program, and any CpGs covered by the first sequencing read were ignored in the second sequencing read in paired-end sequencing [ 20].
Expression was decreased by >80% with the first sequence (A) and by a similar amount with a second shRNA (C) (Fig. 1D).
A second sequence has arisen by mutation and, purely by chance, it resembles the first sequence; therefore, it also binds the transcription factor.
Let us assume as a null hypothesis that the function of the new gene is not altered by the elongation of the first sequence.
Hence, the typical pattern created by gene conversion is: a prefix of sequence h followed by a short internal fragment of a sequence h′, which is then followed by a suffix of the first sequence h.
This is the first sequence by which low discrepancy was formed.
A second sequence was then generated from the first sequence by substituting nucleotides with a probability corresponding to the substitution frequency d, as calculated with Jukes-Cantor.
This is the first sequencing of a horse genome by next-generation sequencing and the first genomic sequence of an individual Quarter Horse mare.
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