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In particular in some deformation processes an instability of geometrical nature is observed when the second gradient model is used, that can be suppressed by the first gradient contribution.
Water molecules will be dephased by the first gradient and rephased by the second gradient.
In case of molecules that move in the direction of the gradients, the phase shift created by the first gradient will not be rephrased by the second gradient.
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By eliminating the spectrum for the first gradient increment, we were able to drastically attenuate the water peak, permitting the measurement of the diffusion coefficients for the nearby H-4 and anomeric proton resonances.
The refocusing introduced by the second gradient will be visible on MR diffusion images as high signal.
Moving spins however acquire an effective phase shift because their motion limits rephrasing by the second gradient.
However, mobile water is not completely rephased by the second gradient due to movement to a different microenvironment during the application of the first gradient, so that a subsequent reduction in signal intensity is observed[ 6 8].
In stationary water molecules that have not moved substantially between the first and second gradient, the extra rotation induced by the second gradient will be identical to the first one and all of them will be in phase at the end of the second gradient, generating virtually a high signal.
The first gradient pulse alters the phase shift of each proton by an amount dependent on the water molecule's spatial location relative to the gradient.
The first gradient pulse induces a phase shift of water molecules which will be followed by an incomplete rephasing after the second gradient pulse, with a magnitude depending on the mobility of the water molecules.
The first gradient causes molecules to acquire phase shifts; the second gradient cancels the gained phase shift by rephrasing, non-moving stationary spins.
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