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The goal was to focus on the constraints placed by the external gradient noise.
As per Fig. 1, we indicate that proto membranes, proto nucleic acids (genetic code) and proto-proteins co-evolved chemically as a system of reaction patterns coupled and driven by the external gradient of temperature, water activity and electromagnetic radiation.
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The external gradient of α-factor resulted in an internal gradient of active G-proteins (Gα-GTP and Gβγ).
Amplification refers to the conversion of the external gradient signal into the polarization of the internal component.
In addition, we introduce a coupling between the activity of the receptors in the external gradient and the MT dynamics.
Accordingly, this duration is diffusion-limited and does not depend on the characteristics of the external gradient (Figure 4F).
A pure cooperativity model (h = 0, no receptor polarization) could not sufficiently amplify the external gradient (Fig 7A).
In particular, receptors can diffuse back to a non-polarized distribution when the external gradient is switched off [24].
The formation of such a cell polarity presumably serves for signal amplification, by turning a weak external gradient into a steeper internal one.
By bringing more signalling molecules on the leading side of the cell, a shallow external gradient can be potentially turned into a steeper internal one.
For railway axles in service, this microstructure gradient is superimposed to the stress gradient introduced by the external load of rotatory bending imposed on the axle.
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