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Its fluctuation is caused by the experimental errors, individual variation, and sensitivity difference in microarray.
This may be explained by the experimental errors when we centered the inclusion under the probe.
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Both ANOVA and DR analyses are affected by the experimental error, response profile, number of observations and experimental design.
This might be supported by the high inter-patient variance of the measured acylcarnitines, which is caused not only by the experimental error but also by the biological variance between patients.
It can be explained by taking into account the experimental errors not included in the original work.
The proportions of alleles (genome-wide) in the LGS populations surviving 3 mg CQ kg-1 day-1, revealed by LGS-Illumina and by LGS-pyro were remarkably similar (Additional File 3) suggesting that the experimental errors incurred by either methodology were small.
These precautions helped with taking precise readings to avoid the experimental errors suspected by Neuzil (Neuzil 1986).
The experimental errors were estimated by Monte Carlo simulations using the standard deviations of the individual experiments.
Certainly, the presence of false positives cannot be ruled out but, likely, the many individual observations (2640) resulted in increased power and precision by reducing the experimental error [ 41].
Covalent bonding of acetate ions from [C2mim]OAc to biomass [ 21] and the reaction of imidazole cations with saccharides to form imidazole glycosides [ 22] may occur but at the low biomass concentrations used here the magnitudes of these reactions are small by comparison to the experimental error in the measurement of IL recovery.
Therefore, one needs to consider models whose predictions deviate from those of the optimal one by an amount within the experimental error (Saez-Rodriguez et al., 2009).
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