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This was also verified by the evolutionary analysis of ORF5, a putative virulence marker at the amino acid level (Fig. 6B).
Notably, DNA-DGGE profiles (Fig. 1a) fully segregated according to insulin-sensitive (IS) or insulin-resistant (IR) phenotypes, as shown in Fig. 1b by the evolutionary analysis based on Pearson's tree method.
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This paper sets up a background for the evolutionary analysis of cultures by testing the null hypothesis of no evolution.
In addition, we did the evolutionary analysis of SlbHLH genes by comparison of tomato bHLH genes with that of angiosperm, early land plants and algaes.
JMEL performed the evolutionary analysis.
The present study supports the recent origin of self-compatibility proposed by the molecular evolutionary analysis in two respects (Tsuchimatsu et al., 2010).
Three regions of most significant conservation were identified by the EARS (Evolutionary Analysis of Regulatory Sequences) [ 18] method and these were confirmed by either or both of the Clustal and FSA Fast Sequence Alignmentt) procedures [ 18- 20].
A hypothesis of adaptive change that is based on the use of KA/KSvalues can be strengthened by joining the molecular evolutionary analysis to an analysis based on structural biology [ 62, 63].
Given this number of sensitive positions predicted by our evolutionary analysis, the mutation is validated as being responsible for the AI and this region as playing an important role during enamel matrix formation.
To generate the time-calibrated Sordariomycetes tree needed for the CAFE analysis, the Bayesian Evolutionary Analysis by Sampling Trees (BEAST) package v.2.2.1 [ 46] was used.
6) We assessed the rate and time scale of evolution by applying Bayesian Evolutionary Analysis by Sampling Trees (BEAST) in the one patient series with a sufficient density of longitudinal samples and have included that analysis.
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