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We validated a Flow Injection Analysis tandem Mass Spectrometry (FIA-MS/MS) to replace the enzymatic method routinely used for neonatal screening in order to monitor in parallel to Phe, Tyr and branched-chain amino-acids not detected by the enzymatic method.
The serum concentration of creatinine was measured by the enzymatic method with creatininase.
Total cholesterol (TC) was estimated by the enzymatic method as described by [24].
On the 15 sets of frozen samples, the W-I variation of creatinine measured by the enzymatic method (CV 5.2%) was slightly less than by the picrate method (CV 6.2%).
We measured intracellular iron levels by the enzymatic method using Calcein-AM, a fluorescent dye to establish iron-deficient conditions (Fig S2) [50].
Pyruvate and lactate content in the medium was measured by the enzymatic method using a commercially available fluorescence-based assay kit or using the CMA 600 Analyzer (CMA-Microdialysis) as described previously.
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Plasma total cholesterol (TC), triglycerides (TG) and HDL-cholesterol (HDL-C) were assessed by the enzymatic methods (Cormay, Warsaw, Poland).
Plasma total (TC) and HDL-cholesterol (HDL-C) and triglycerides (TG) were assessed by the enzymatic methods (Cormay, Warsaw, Poland).
Serum total and HDL cholesterol and triglycerides (TG) were assessed by the enzymatic methods using commercial kits produced by ANALCO-GBG, Poland.
SCr and urine creatinine (UCr) concentrations were measured using the enzymatic method by the dedicated researcher.
Significant differences were found in the results obtained by the Folin-Ciocalteu and enzymatic methods indicating that the enzymatic method needs further standardization [ 25].
More suggestions(17)
by the enzymatic activity
by the enzymatic system
by the same method
by the enzymatic cholesterol
by the enzymatic removal
by the trapezoidal method
by the enzymatic cycling
by the enzymatic activation
by the enzymatic assay
by the scientific method
by the enzymatic isoform
by the enzymatic digestion
by the enzymatic reduction
by the enzymatic hexokinase
by the enzymatic complex
by the horizontal method
by the enzymatic reaction
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