Perlin's conceit, while amusing, is slight, and it isn't bolstered much by the show's other entry, "Sequence".
The transfer vector contains the mutated PrP sequence, followed by an internal ribosome entry sequence (IRES), and the enhanced green fluorescent (GFP) protein marker gene (Figure 1A).
Phosphorylation site (Ser/Thr to Ala) mutants of human TSC2 were generated using QuickChange Multisite-directed Mutagenesis (Stratagene), sequence verified, and subcloned into a previously described retroviral vector (IRES-Hygro, gift of D.J. Kwiatkowski) upstream of an internal ribosome entry sequence followed by the hygromycin-resistance selectable marker [55].
Our Backward PIN-Sequence Inference algorithm exploits the inherent physical constraints between key entries to infer the complete user key entry sequence.
To minimize interference with DAT function by preservation of both DAT alleles, Cre recombinase expression was driven from the 3'-untranslated region (3'UTR) of the endogenous DAT gene by means of an internal ribosomal entry sequence (IRES).
Briefly, after removal of the mElf5 and eGFP transgenes, PyV mT cDNA was sub-cloned between the Tet-operator (TetO) and internal ribosome entry sequence (IRES), followed by sub-cloning of Cre recombinase cDNA downstream of the IRES (TetO-PyV mT-IRES-Cre recombinase; abbreviated as MIC) (Additional file 1: Figure S1).
The plan, designed by Machado and Silvetti Associates, also created a new entry sequence that leads visitors from a four-story parking garage up to the crest of a hill, where they follow a path alongside the villa before descending back down through the amphitheater to the main entry.
When annotating an InterPro entry, a curator first identifies those UniProtKB/Swiss-Prot (i.e. reviewed) sequences matched by the entry that has been experimentally characterized.
If a UniProt entry sequence is a substring of a longer sequence, the entry is removed and the entry with longer sequence is kept.
