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The final topology of membrane proteins is thought to be dictated primarily by the encoding sequence.
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Then, we calculated the correlation coefficient for the encoded sequences by using (4).
To clone pQHA-puro, the Flag encoding sequence was replaced by the HA encoding sequence by ligating the annealed oligonucleotides GM68 and GM69 with MfeI- and AgeI-digested pQFlag-puro.
Using primers which were extended by 9 nucleotides encoding SKL the protein encoding sequences of An02g07020, An09g03100, An09g06390, An11g03120 and An14g05380 were modified at the C terminus.
RPmt is targeted to the mitochondrial matrix by the sequence encoding the N-terminal 12 amino acids presequence of subunit IV of cytochrome c oxidase.
In human populations, codon 472 of KDR is encoded by the nucleotide sequence CAA, which encodes glutamine, or CAT, which encodes histidine.
In human populations, TP53 codon 72 is encoded by the nucleotide sequence CCC, which encodes proline, or CGC, which encodes arginine.
By encoding the sequences using the reduced amino acid alphabet information, a predictor was developed to identify the four different families of J-proteins in the present work.
Third, we examined the length-distributions of LS indels in coding regions, considering (a) all LS indels in the ENCODE regions and (b) only those that are supported by the comparative-grade ENCODE sequences.
Mora et al. demonstrated that the data from Weinstein et al. prove that "antibody diversity is not limited by the sequences encoded in the genome" [ 40].
The plasmid encoding the FLAG-tagged m-IL-1β was constructed by deleting the sequence encoding AA2-117 of pro-IL-1β and inserting a DNA sequence encoding a single FLAG before the stop codon by PCR-based mutagenesis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com