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After cloning, DNA fragments were sequenced by the dye terminator method (PRIMM Facility, Naples, Italy).
The purified PCR products were directly sequenced by the dye terminator method (PRIMM Facility, Naples, Italy).
PCR products were sequenced by the dye terminator method and with an automatic sequencer.
PCR-amplified DNA was sequenced by the dye terminator method in both the forward and reverse directions.
The purified PCR product (535 bp) was directly sequenced by the dye terminator method (PRIMM Facility, Naples, Italy).
The purified PCR product (573 bp) was directly sequenced by the dye terminator method (PRIMM Facility, Naples, Italy).
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The sequencing reactions were performed by using the Dye Terminator cycle sequencing kit with AmpliTaq DNA polymerase FS (Applied Biosystems) and the automated 373A NA Sequencer (Applied Biosystems).
Sequencing of PCR products was performed by using the Dye Terminator Cycle Sequencing Quick Start Kit (Beckman Coulter, Fullerton, CA, USA).
SNP genotyping was by the dye terminator-based SNaPshot method (Applied Biosystems, Warrington, UK).
Some of the sequence changes were then validated by the dye-terminator method.
All chromatograms produced by the dye-terminator sequencing method have a certain amount of "background noise," small spurious peaks that do not represent true bases.
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