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Under general anesthesia, indocyanine green (1 ml) was injected into the periareolar skin and periphery of tumor for SLNB by the dye method.
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Protein determination was performed by the Bradford dye method, using the Bio-Rad reagent and BSA as the standard.
Protein concentration in the supernatants was determined by the Bradford dye method using Bio-Rad reagent.
Cells were lysed in lysis buffer and the concentration of protein was determined by the Bradford dye method (Bio-Rad Laboratories).
Protein determination was performed by the Bradford dye method.
Protein concentration was measured by the Bradford dye method (Bradford 1976).
The velocity values measured by the Pulse Boundary Model were about 20 50% lower than those estimated by the dye tracer method although they were strongly linearly correlated.
Protein concentrations were determined by the dye binding method of Bradford Bradfordd, 1976 ) using the Bio-Rad Protein Assay Kit (Bio-Rad LaBovinerieserum).. Bovine serum albumin (BSA), in concentrations of 0.1 – 1.0 mg mL-1, was used for the standard calibration curve.
Cell number was measured by the Dye uptake method using Neutral Red cell incorporation.
The purified products were sequenced on an Applied Biosystems 3730 automated DNA sequencer by the dye termination method.
The purified PCR product (535 bp) was directly sequenced by the dye terminator method (PRIMM Facility, Naples, Italy).
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