Sentence examples for by the dye binding from inspiring English sources

Protein concentrations were determined by the dye binding method of Bradford Bradfordd, 1976 ) using the Bio-Rad Protein Assay Kit (Bio-Rad LaBovinerieserum).. Bovine serum albumin (BSA), in concentrations of 0.1 – 1.0 mg mL-1, was used for the standard calibration curve.

Urine protein was measured by the dye binding method using a complex of pyrogallol red and molybdenum acid (QuanTtest Red Total Protein Assay System, Quantimetrix, Redondo Beach, CA).

The protein concentration was determined by the dye-binding method [ 18].

Protein concentrations in the supernatants were determined by the dye-binding method according to manufacturer's instructions (BioRad Laboratories, Hercules, CA).

The soluble and immobilized protein concentration was determined by the dye-binding procedure of Bradford using bovine serum albumin as a standard, as described previously [ 10].

Protein concentrations were determined by the dye-binding method of Bradford using a prefabricated assay from Bio-Rad Laboratories (HerCAles, Candand bovine serum albumin as calibration standard.

Protein contents of crude preparations or partially purified fractions were determined by the dye-binding method of Bradford using a pre-fabricated assay (Biorad Laboratories) and BSA as calibration standard.

The protein concentrations of CTec2 and HTec2 were determined to be 130 mg/ml and 101 mg/ml, respectively, by the dye-binding assay of Bradford [ 19] using bovine IgG as standard.

One unit of PPO activity was defined as the amount of enzyme that caused a change in the absorbance of 0.001 per min. The protein concentration of the extract of green pea seeds was determined by using the dye-binding method of Bradford [ 18]; bovine serum albumin was used as standard.

The protein concentration of the supernatants was determined by the Bradford dye binding method.

To normalize the cytokine measurements to total protein, we diluted the supernatants to 2 mg/mL in phosphate-buffered saline (PBS), and total protein was assayed by the Coomassie dye binding assay (Pierce, Rockford, IL) with bovine serum albumin as the standard.