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The speed of the erodent particles was measured by the double disk method.
The presence of an extended-spectrum β -lactamase (ESBL -producing strain was dESBL -producingdouble distrainergy test [ 14].
The ESBL phenotype was detected by the double disk (Oxoid, Basingstoke, Hampshire, England) synergy test (DDST) according to CLSI [ 12] guidelines.
For suspected ESBL-producing K. pneumoniae isolates, ESBL production was confirmed by the double disk synergy test according to the CLSI performance standards [ 17].
Of the 84 isolates screened for ESBL production by the double disk test, 29 (35.5%) were positive using either cefotaxime or ceftriaxone.
For the available 'suspected ESBL-producing K. pneumoniae' isolates, ESBL production was determined by the double disk synergy test according to the CLSI performance standards [ 26].
Similar(51)
Detection of inducible erm gene-mediated clindamycin-resistance (MLSB phenotype) was performed by using the double disk diffusion method as described in M100-S14 NCCLS 2004 Informational Supplement (23 ).
Isolates of both CMRSA-5 and CMRSA-2 related CMRSA-2 relatedre erythromycin-restrains were found thate inducibly resistant to clindamycin were challenged by using the double disk approximation test.
Transconjugants were selected on Luria Bertani/ lysogeny broth (LB) agar plates supplemented with 30 μg /mL cefotaxime and 300 μg /mL rifampicin and were confirmed for the presence of ESBL genes phenotypically, using the double disk synergy test (DDST) disk approximation method and genotypically, by testing for the presence of the respective ESBL gene via PCR.
For the double disk diffusion (D-test), a clindamycin disk was placed 2 cm apart from the erythromycin disk.
ESBL production was tested using the double disk synergy test.
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by the double standard
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