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Conflicting reports regarding the extent to which adult, somatic cells are rejuvenated may also be explained by the distinct protocols and materials used.
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We then selected specific experimental conditions and used a GeLC-MS/MS proteomic approach, where biological samples are separated by SDS-PAGE, sliced, digested in-gel and analyzed by LC-MS/MS, on the DIM fractions retrieved from the two distinct protocols.
Hierarchical clustering analysis provides a global examination whether biologically relevant expression signatures are consistently measured by distinct protocols.
The compounds were tested in the wedge model using two distinct protocols; including baseline stimulation at 1-Hz followed by a brief period at 0.5-Hz, either without an additional brief period of 2-Hz stimulation (i.e. Protocol 1) or with 2-Hz stimulation (i.e. Protocol 2).
Both vectors used in the present work were already tested on Schistosomiasis, using distinct protocols by diverse groups.
There are two distinct protocols for awarding the Medal of Honor.
Dried 2-AB-labeled glycans were recovered in acetonitrile/water (96:4; v/v) and analyzed following two distinct protocols differing by column formats and detections used, i.e. using a classical normal-bore column for fluorescence detection and using a capillary column for both fluorescence and mass spectrometry detections.
DIMs were further isolated from the root microsomal fraction according to two distinct protocols.
To the best of our knowledge only three distinct protocols have been reported so far.
Why did the authors not perform neural and mesodermal differentiation in parallel using two distinct protocols?
We treated MALC via two distinct protocols.
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