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Of the three protein methods tested, the maximum likelihood method scales the best, followed by the distance method, and then the maximum parsimony method.
The fact that the results obtained by the distance method are not perfect can be attributed to the reconstruction algorithm Neighbor Joining (see Gascuel [ 37]).
We therefore limit the search to the topologies present in the posterior, and designate this approach by a lowercase 'm' followed by the distance method (mRBS, mRAS, etc).
For detection of recombination events, the closeness between the suspected recombinant and reference sequences was measured as bootscanning supports by the phylogenetic method, and as sequence similarity by the distance method.
In 10 concatenations where the order of the two alleles of each intron was randomly changed, the resulting trees were similar among each other and similar to the tree obtained by the distance method (Additional file 1: Figure S2), therefore also supporting the same group assignment.
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By contrast, the distance method seemed to be oversimplified and couldn't provide such information.
Phylogenetic analyses were conducted with the MEGA version 3.1 software (26 ) by using the distance method and the neighbor-joining algorithm with Kimura-2 parameters.
In this latter case, the variations mainly arise from the placement of V. cholerae, either at the root of Gram- or E. coli/ S. typhimurium clades By contrast, the distance method leads to variable results: no distance tree has a d T lower than 6 when compared to the consensus tree.
For phylogenetic analysis by the distance methods, we used programs in the Phylip 3.65 package (http://evolution.gs.washington.edu/phylip.html).edu/phylip.html
In addition, it is not affected by the centroid like the distance method and the area method that fail in ranking fuzzy numbers having the same centroid (Deng et al. 2006).
The result of K2P can be explained by the fact that the distance method uses exactly the model used to generate the data.
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