This discrepancy may be attributed to the different quantification analyses by which that study assessed only the expression intensity of YKL-40 in cancers.
The agreement between the results obtained with the two different quantification methods was evaluated by the Bland-Altman analysis including a linear regression approach [18, 19].
The differences in biological matrix (i.e., capillary versus venous data) might be explained partly by different quantification methods (i.e., HPLC-UV and LC-MS/MS).
In contrast to changes in levels of Armadillo induced by the different mutant forms, quantification supported the observation that E-Cadherin levels change little in the AJ upon mutant overexpression (changes of less than 10% are not shown), with the exception of ΔNArm1 155, which caused a 40% decrease in E-Cadherin levels (Figure 12).
Many, but not all studies have reported a reduction in the number of neurons, particularly cholinergic neurons in aged animals, but comparison of the data is complicated by the different approaches for quantification of neuronal numbers used [see (Kapur 2013; Saffrey 2013)].
The BYDV titre was determined in two oat varieties by RT-qPCR using three different quantification approaches.
The nature of the physical activity questions did not allow a quantification of physical activity by the different domains.
The data of the six 28-samplestandard curves were used to generate 61,236 and 244,944 different quantifications by the 2ΔΔCq-approach and based on external standard curves, respectively.
This may be due to the fact that the expression might be very low and thus be undetectable by immunodetection, as suggested by the relative quantification of the different transcripts.
Both of these methods score the smears by quantification of the different vaginal morphotypes, making the evaluation of smears very subjective that requires an experienced slide reader and also considerable time and skill.
