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The cells, grown to 70% confluence and treated for 48 h, have been then trypsinized or treated as requested by the different protocol of the experiments and the biological parameters evaluated.
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Processing of pre- to post-gel was not influenced by the different protocols.
The collectors and aggregators are provided by the different protocols of the system, identified by a protocol owner id.
We strive to uncover the requirements considered by the different protocols, the resource limitations under which they operate, and the design decisions made by the authors.
In particular, to have a fair comparison, we observed the detection time provided by the different protocols using the same energy budget.
Hence, the literature is contradictory on the implication of ASCs in tumor growth and that can be partially explained by the different protocols used both in vivo and in vitro, which makes it hard to compare them.
Table 1 summarizes the use of the different protocols by participant.
Of those, nearly all genes displayed higher expression levels for TriZol versus Qiagen RNA and were expressed at low to medium levels (<10 rpkm) (Additional file 3: Table S2).> -wrap-foot> tableablistssthethe number of protein coding genes detected as differentially expressed by the NOISeq algorithm across the different protocols.
However, when comparing the same samples sequenced by the two different protocols, there are many genes that are far away from the diagonal lines along the length of the axis in Fig. 6.
The samples are clearly clustered by sequencing protocol, and while the correlation for samples prepared with the same protocol is nearly 1, the correlation for samples sequenced by the two different protocols is around 0.93.
The differences can be explained by the different experimental protocols and the biological differences between the nude and SCID mice.
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