Sentence examples for by the dideoxy terminator from inspiring English sources

The DNA of each amplicon was sequenced by the dideoxy terminator method on an automated DNA sequencer (3100 Genetic Analyzer, Applied Biosystems) based on capillary electrophoresis.

PCR products were cloned in plasmids and sequenced with the dideoxy terminator technique.

The LMR-12 cDNA clone was sequenced using the dideoxy Terminator Cycle Sequencing Kit on an automated 373A DNA sequencer (Applied Biosystems Benelux B.V., Maarsen, The Netherlands).

Prepared plasmid DNA was then sequenced by the dideoxy termination method [ 25].

In bisulfite sequencing, polymerase chain reaction (PCR) amplification of converted DNA is used to replace the uracil with thymine, and the PCR product is analyzed by Sanger dideoxy terminator sequencing (Zilberman and Henikoff 2007).

The clones were sequenced from both ends by the dideoxy chain termination method using BigDye Terminator Cycle sequencing V2.0 Ready Reaction (Applied Biosystems).

Amplified samples were then sequenced by the dideoxy chain termination method using ET terminator chemistry using a MegaBACE 500 instrument and Cimarron 3.12 basecaller (GE Healthcare, Amersham plc., Buckinghamshire, UK).

For approximately 30 years following its original publication in 1977 [ 2], the Sanger dideoxy terminator method was the standard for DNA sequencing.

Both strands of the plasmid DNA were fully sequenced downstream of an insert site by the dideoxy chain-termination method using a BigDye® Terminator v. 3.1.

PCR fragments were isolated from 1-1.51-1.5%ose gel using the QIAquick gel Purification Kit (cat. number: 28704) and sequenced by a cycle sequencing reaction by Sanger's dideoxy Terminator Method on a PCR Machine.

Sequencing of the purified DNA was performed using the Taq Dideoxy Terminator cycle sequencing kit and an ABI Prism 377 DNA sequencer (Applied Biosystems).