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In some cases, the functional state represented by the determined structures is not straightforward to characterize.
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The structure was solved by molecular replacement using the previously determined structure of the non-glycosylated form of Evasin-1.
Here we show that PTEX is a bona fide translocon by determining structures of the PTEX core complex at near-atomic resolution using cryo-electron microscopy.
The different values of hygroscopicity are corroborated by the structures determined by X-ray analysis: acetamidinium salts with 2D layered structures are not hygroscopic while acetamidinium salts with 3D layered structures are quite hygroscopic.
This notion is supported by the previously determined crystal structure of E. coli OmpW, which shows a lateral opening similar to that of OprG [17].
The data cannot be described by the structure determined by PCSs alone, or indeed by any single structure, thus indicating that the encounter ensemble must represent quite a significant fraction of the complex.
The different values of hygroscopicity are corroborated by the structures determined by X-ray analysis.
The lab has also made fundamental discoveries related to the structural basis for high-speed DNA replication, by determining the structures of the clamp-loader and sliding clamp complexes of DNA polymerases.
Here we report our investigation on the evolution of the AZFc region of the Y chromosome by determining the structure and copy number of the DAZ gene and the conservation of AZFc ampliconic sequences on primate Y chromosomes.
Here, we have explored the issue further by determining the structure of the calcium-free (apo) C-terminal domain of F1 TnC (F1 CTnC) and studying its dynamics in solution.
Bai et al. demonstrate the potential of direct-electron detectors in cryo-EM by determining the structures of two ribosomes.
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