Sentence examples for by the detection objectives from inspiring English sources
The middle and right columns show the fluorescence signal excited with these speckle patterns captured by the detection objectives 1 and 2 respectively.
The images captured by the detection objectives again become blurry due to out of focus fluorescence and the intensity of single beads depends on their overlap with the illuminating speckles.
Therefore, a stack of ∼20 transmission images was taken by sliding the sample through the detection objective's plane of focus.
The light sheet had to be created within the working distance of 280 µm of the detection objective and was produced by a dedicated illumination objective lens with a high NA in order to achieve a thin light sheet with an axial extension of only 3 µm full-width-at-half-maximum (FWHM).
Two objectives, perpendicular to the detection objective, were used for double-sided SPIM illumination.
The focal plane of the detection objective was imaged onto an sCMOS camera (Zyla, Andor).
One can switch between the images coming from the two detection objectives via a removable mirror.
The fluorescence generated within the specimen is collected by a detection objective (Nikon, CFI Apo LWD 25XW, 1.1 NA, 2 mm WD) whose focal plane is co-incident with the light sheet.
The leftmost column shows the illumination speckle patterns behind scotch tape observed by detection objective 1.
The localization of H. felis-infected mucosa at 8 weeks was estimated by the detection of bacteria using microscope with × 100 objective.
Molecular imaging with targeted tracers by positron emission tomography (PET) allows for the noninvasive detection and characterization of biological changes at the molecular level, leading to earlier disease detection, objective monitoring of therapies, and better prognostication of cardiovascular diseases progression.
