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Thus, the illumination frequencies are limited by the detection bandwidth.
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The pump beam is frequency shifted by 150 MHz using a fiber-coupled AOM (Gooch & Housego) to shift spurious interference between pump and probe beam outside the detection bandwidth of the MTS signal.
The detection bandwidth was set at 488 nm for 5-methylcytosine indicator, and fluorescence intensity of the nucleus was recorded (N = 20 per layer per case).
The detection bandwidth is equal to the inverse of the frame exposure time, which is 10 msec for these data.
Again, the −6 dB roll-off point is found to be at ~950 MHz close to the detection bandwidth.
The detection bandwidth scales inversely with the sampling time, which for a minimum of a 125 ns acquisition time corresponds to 32 MHz of frequency bandwidth about the center frequency in the underlying electronics.
Alexa 488 was excited using an argon laser (488 nm), and the detection bandwidth was set at 493 to 601 nm (expected peak at 520 nm).
The SNR of any shot-noise limited LCS system is given by Here, ε is the detector quantum efficiency; hv is the photon energy and fBW is the electronic detection bandwidth of the system [ 5].
Because of the extremely wide detection bandwidth of the optical micro-ring resonator [ 30], we expect that the axial resolution can be significantly improved.
Due to detection bandwidth limitations, the true depth of the −6 dB point might actually be higher.
We show that the FPD, which represents the average fluorescence lifetime of fluorophores at any given detection bandwidth, is inherent to all cytometry systems and proved this by simulation and evaluation with different algorithms.
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