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All the nerves on the surface of the peritoneal sheath show GFP fluorescence driven by the dTdc2 driver (Fig. 5D).
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It allows random labeling of single cells from the ensemble of cells visualized by the GAL4 driver line.
Here we employed immunocytochemistry to test the effect of targeted sNPF and DTK knockdown in the ipc-1 cells by the Kurs6 Gal4 driver.
When PACα was expressed by the Or83b-Gal4 driner in Or83b-/ flies, and the fly antennae were exposed to blue light, additional neuronal activity could be recorded (Figure 6G).
Their expression is driven by the 24B-GAL4 driver which induces GAL4 expression during the early phases of mesoderm development and maintains expression in the musculature throughout embryogenesis.
For example, expression of W557S by the Act5C driver caused lethality, yet Lamin C protein levels were nearly equal to that of endogenous Lamin C and to that produced by the ey driver, which allowed for viability (Fig. 7).
In addition, the relative levels of N210K and the N terminal truncation produced by the Act5 driver were nearly equivalent, yet only the N-terminal truncation caused lethality (Fig. 7).
In the absence of TSA, wings overexpressing Gro driven by the MS1096-Gal4 driver show 26% severe blistering, 11% moderate blistering, 47% mild blistering and 16% no blistering (Fig. 4E, purple bars).
High level overexpression of Gro in the dorsal wing pouch (directed by the MS1096-Gal4 driver) leads to wing blistering, likely due to improper adhesion between the dorsal and ventral epithelia that comprise the wing blade [31].
Co-expression of AOX in the same tissues via UAS-AOX rescued the lethality of Cox5b knockdown by the G14 driver (Fig. 4B).
They also depend on tGgIdas transcription since they are found absent when it is not activated by the Gal4 driver (data not shown).
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