The syringe was connected to the MEA dish by a narrow tube, at the end of which was a needle, inserted and stabilized by a rubber connector placed within the custom built cap of the MEA.
By using the custom built animal assay stage and the automated data analysis, this system has been optimized to have reasonable throughput for supporting routine animal studies in drug discovery.
Unmapped reads may potentially present a spliced alignment on the genome easily detectable, again using Bowtie, by mapping to a custom built splice junctions library.
Additionally, the cell under study was continuously superfused by means of a custom built close delivery system with a pipette of tip diameter 200 μm placed ∼300 μm from the cell.
The MEA dish was continuously perfused with feeding media (described above) at a rate of 2.5 ml/day by means of a custom built perfusion system based on an ultra-slow flow peristaltic pump (Instech Laboratories Inc., Plymouth Meeting, PA, USA) using an imbalanced set of silicone tubes.
A sudden release of the load was achieved by a custom built electromagnetic mechanism.
Second, each subject scan was accompanied by a scan of a custom built phantom that was centrally evaluated for signal-to-noise and geometrical fidelity to ensure performance of the MRI scanners remained within a specific tolerance limit.
Giancarlo Stanton was among the first to don a helmet capable of blocking pitches bound for the face after he was hit by Mike Fiers in 2014, but the custom build hasn't caught on.
In the current study, the tone (CS, 400 ms duration) was generated by a custom built sound generator (Department of Electronic Engineering, University of Goettingen) and presented through earphones (Cherry Inc, Japan) at an intensity perceived as very loud by each participant, usually 80dBB.
This level of sophistication increases the accuracy of the experiment and is increasingly the kind of thing that faculty don't need to have custom built by software programmers.
"This was followed by a period of scraping images with a custom built scraping tool, by Adam Harvey, for Google and Flickr," notes Dullaart.
