Sentence examples for by the control construct from inspiring English sources

A comparison of the maximum fluorescence (ΔF/Fo max) at +90 mV (Fig. 6B) shows that the signal generated by fs-α1S was significantly smaller than that generated by the control construct (wt-α1S vs. fs-α1S t-test significance p = 0.014) and smaller than that generated by the two coexpressed fragments (fs-α1S vs. fs-α1SM701 fs-α1SM70100 t-test sIgnificance p = 0.013).

Vector control lines were verified by PCR to contain the control construct (data not shown).

Complete cleavage was verified by a PCR amplification of the control construct insert with 1  μl of the HpaII or MspI digestion reactions (10 fg plasmid DNA) under standard conditions.

If the control construct were to deplete R15 from its normal location and if this were to impair its enzymatic activity, the observed effect of mDia2 induction would be minimized since eIF2α phosphatase activity would have been impaired similarly by the GFP-R15B 1-146 constructonstruct (expressed at similar levels to the mDia2 construct on western blots).

The D6 element reduced the expression of the pGL3 control construct by ~50%, confirming that this element represents a repressor.

Expression of (GR 80, but not the (GR 80 control construct, by Vg- Gal4, which drives gene expression in the dorsoventral boundary and some other cells of the wing imaginal discs [ 11], gave rise to wing margin defects in 90%% of flies of both sexes at 25 °C (Fig.  2c, d).

The RFP control construct was generated by fusing RFP with the gpdA promoter [43] and cloned into the EcoRI site of pJW53 giving rise to pNI21.

Transcription from the DAP-A and DAP-G construct was suppressed by 39%and70%0%, respectively, compared to control construct no. 1.

A control construct was created by inserting a stop codon after the TAP tag sequence.

Relative cell densities were compared with cells containing an empty vector control construct and were quantified by SRB assay.

The magnitude of the fluorescence signal expressed by fs-α1S was approximately 5-fold larger than the largest Ca2+ transient detected in non-transfected myotubes expressing Idys, >20-fold larger than the average Ca2+ transient detectable in non-transfected cells, and about 1/3 of the maximum SR Ca2+ release expressed by the control wt-α1S construct.