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Highly mutated viruses can no longer replicate, and thus technology for producing viral vectors by the complementation of defective genes using complementing cells or packaging systems is impeded by low vector yields and contamination with wild-type virus rescuants.
The classical dominance model of heterosis explains the superiority of hybrid plants by the complementation of deleterious parental alleles by superior alleles of the second parent at many loci [3].
The potential functional overlap between these CRTs was corroborated by the complementation of AtCRT1b mutants by AtCRT1a.
To test if FT chromatin is directly targeted by ELF6 and AtJmj4, we preformed ChIP assays using transgenic plants expressing functional ELF6::GUS [22] and AtJmj4::FLAG (Figures 4B and 4C) as demonstrated by the complementation of the elf6-4 and atjmj4-1 mutant phenotypes, respectively.
The cDNA of Ae.CSAS was amplified, cloned, and functionally evaluated by the complementation of CSAS-deficient LEC29.Lec32 CHO cells.
Indeed, it is worth exploring the biological significance of a stable physico-chemical framework of amino acids by the complementation of GC-dependent amino acids.
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Both phenotypes were complemented by pKTH3832, indicating that they are caused by the Ecs deficiency (the complementation of the lysostaphin sensitivity is shown in Figure 1C).
The full-length enriched cDNA clones will enable the construction of overexpressing mutant plants by introduction of the cDNAs driven by a constitutive promoter, the complementation of Thellungiella mutants, and the determination of promoter regions in the Thellungiella genome.
In spite of the high conservations between the catalytic domain of all CDC14 phosphatases [ 5] and the complementation of ScCdc14 by hCDC14B [ 6], human CDC14s have so far been reported to be involved in functions that are quite diverse than that of budding yeast [ 7].
In vivo function of the L. major hemG gene was shown by the functional complementation of the Escherichia coli ΔhemG strain LG285.
In the absence of a functional enzymatic assay for PpIRX10 it is currently not possible to be certain about its enzymatic activity, although it is reasonable to hypothesise that it plays a role in xylan biosynthesis, a conclusion which is supported by the partial complementation of the Arabidopsis irx10 irx10-L double mutant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com