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MiRNAs are generated from hairpin structures formed by the complementary sequences within one transcript.
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The halves of DNAzyme were connected by the complementary sequence of aptamer.
This design leads to a lower Tm value and thermodynamic energy, so that the reporter DNA is likely to be replaced by the complementary sequence of the target mRNA, leading to more stable hybridization.
MicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression post-transcriptionally by binding to the complementary sequences of targeted mRNAs.
MicroRNAs participate in post-transcriptional regulation of cellular differentiation by binding to the complementary sequences of target messenger RNAs (mRNAs) or non-coding RNAs and down-regulate their target gene expression.
Given that kinase and substrate have complementary sequences in a 3D space, stabilization of the activation loop in a certain position may modulate substrate preference by shifting the interactions of the complementary sequences.
The second round of PCR joins the 5′ flank (truncated pacC gene) or 3′ flank with the partial hph coding sequences amplified in the first round, by annealing of the complementary sequence present in the reverse primer of pair 1 or the forward primer of pair 2, respectively.
The hybridization events were evaluated by ECL measurements and only the complementary sequence could form a double-stranded DNA (dsDNA) with DNA probe and give strong ECL signals.
The reverse primer (5′-CAT GGATCCCTATTCTGACGATCC-3′) is designated primer R1 and contains a BamHI restriction site (in bold) followed by 15 bases corresponding to the complementary sequence of the cg10062 gene.
The reverse primer (5′- CC TTC CGG AAG CTT ACC AAC TTT TTT CTG AAA TGT GAC C -3′) contains a HindIII restriction site (in bold) followed by 25 bases corresponding to the complementary sequence of the Ch-MAL gene.
In PNA-mediated PCR clamping, PNA oligomers suppress the amplification of the complementary sequence confined by a pair of DNA oligonucleotide primers because PNA are not substrate for DNA polymerase.
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