Sentence examples for by the comparative threshold from inspiring English sources

Relative quantification of the mRNA copies in the Ts1Cje samples compared to that of the WT samples was performed by the comparative threshold cycle method using Microsoft Excel.

The relative abundance of transcripts was calculated by the comparative threshold cycle (CT) method.

SYBR Green real-time PCR was used to quantify CHS mRNA expression by the comparative threshold cycle method (User Bulletin #2; Applied Biosystem, Foster City, CA).

Relative quantities of Bcl-2, Bcl-xL, Bax, Bak, LC3A, LC3B, LC3C and beclin-1 mRNA, and 18S ribosomal RNA were calculated by the comparative threshold cycle (Ct) method as previously described [20].

Quantitative Real-time PCR was performed in a StepOne Plus Real-Time PCR System using TaqMan gene expression assays for VCAM-1 and GAPDH with TaqMan Gene Expression Mastermix (Applied Biosystems, Warrington, UK) Data were generated by the comparative threshold cycle (ΔΔCT) method by normalizing to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).

The thermocycling protocol was: 95°C for 3 min, 40 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Collected data were analyzed by the comparative threshold cycle (CT) method using RPS18 expression as an endogenous normalization control.

Expression profile in each specimen was assessed by using the comparative threshold cycle (2-ddCt) method.

Quantitative values were obtained by using the comparative threshold cycle (ΔΔCT) method, as described in Livak et al. [98].

The relative expression of each gene was determined by using the comparative threshold (Ct) method [ 27].

Relative quantity of target cDNA was computed by using the comparative threshold (Ct) method.

The relative occupancy of the immunoprecipitated factor at a locus was estimated by using the comparative threshold method.