Sentence examples for by the comparative cycle from inspiring English sources

Relative quantification was performed by the comparative cycle threshold method [39].

Relative expression levels of targets were calculated by the comparative cycle threshold (Ct) method outlined in user bulletin no. 2 provided by Applied Biosystems.

Relative fold enrichment was determined by the comparative cycle threshold (CT) method, detecting Ct difference between a target and a standard region of interest [45].

Relative quantification of all targets was calculated by the comparative cycle threshold method outlined by the manufacturer (User Bulletin No. 2; Applied Biosystems).

Quantification was performed by the comparative cycle threshold (Ct) method [ 32].

Results were normalized using 5 housekeeping genes and analyzed by the comparative cycle threshold method.

The hemizygous or homozygous status of transgenic mice was determined by relative real-time PCR, taking a hemizygous DNA as a reference, by using the comparative cycle threshold method [ 15, 33, 34].

By using the comparative cycle threshold method, all data were normalized to endogenous reference gene β -actin and then compared with appropriate controls for calculation of fold change.

Relative quantification of all targets was calculated by using the comparative cycle threshold method [ 24].

Expression relative to GAPDH was calculated by using the comparative cycle threshold (CT) method [ 41].

The relative expression of the genes of interest was normalized against the GAPDH housekeeping gene by using the comparative cycle threshold (Ct) method.