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Amino acid sequences encoded by the coding nucleotide regions of non-synonymous SNPs in the stress-responsive genes were analyzed using Pfam software [ 76] to determine the presence of functional domains/protein families within the genes.
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The aminoacid sequences encoded by the predicted coding nucleotide regions of the microsatellite carrying unigenes were analyzed using the software Pfam [ 38] to determine the presence of functional domain/protein family within the UGMS.
The binary hierarchical clustering dendograms in Figure 4 also further revealed the tendency for the monocot species to be more closely related to each other than to the dicot Arabidopsis in terms of cis-regulatory information content, mirroring the exact same trend established by the coding sequence (nucleotide and amino acid levels) phylogeny.
Two test sequences were generated, one by concatenating the protein coding nucleotide sequences of all genes containing an insertion from our set, the other by concatenating the corresponding sequences for all deletions from our set.
The protein coding nucleotide sequences of GCN5 and PCAF were aligned by using MUSCLE built in MEGA6 (21).
Based on the bacterial genetic code we classified each coding nucleotide by the consequence of the three possible mutations.
We write the Bayes factor between SC0 and as: (27) where A corresponds to the data, composed by an alignment of coding nucleotide sequences and a topology and (28) Here we compute Bayes factors by thermodynamic integration (or path sampling) as described in [ 3].
We have genetically characterized TBEV isolate Glubinnoe/2004 by determining its complete coding nucleotide sequence and comparing it with most of the available TBEV sequences.
After removing the overlap with repeats identified by RepeatMasker, the percentage of coding nucleotides in the three libraries ranged from 3.5% for the Mbo library to 4.6% for the HBa library (the 'coding unmasked' category in Figure 2).
When referring to specific mtDNA nucleotide pair sites in this paper, the coding strand nucleotide is first denoted, followed by a colon and then the nucleotide on the noncoding strand.
With respect to the value of sensitivity at the nucleotide level, Table 2 shows that 93%% of the really coding nucleotides were correctly predicted as such by our program.
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